Enzyme-linked fluorescent assay (ELFA) for the determination of human Procalcitonin (PCT) in human serum or plasma (lithium heparinate) on the VIDAS instruments (bioMérieux).
Assay characteristics
The VIDAS B·R·A·H·M·S PCT assay is a one-step immunoassay sandwich method with a final fluorescent detection (ELFA) can be distinguished by these characteristics:
| Sample matrix | Human serum or plasma (lithium heparinate) |
| Sample volume | 200 μL |
| Incubation time | 20 min |
| Measuring range | 0.05-200 μg/L |
| Limit of Quantification (LoQ) | 0.05 μg/L |
| Limit of Detection (LoD) | 0.03 μg/L |
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Limit of Quantitation (LoQ) and Limit of Detection (LoD) values are rounded to 2 decimal places in alignment with the reported results for the assay. |
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