Assay characteristics
| Test principle |
One-step immunochromatographic assay (sandwich principle) using immunogold labelling |
| Sample matrix |
Serum, plasma |
| Sample volume |
200 μL |
| Incubation time |
30 min at room temperature (18-30 °C) |
| Result |
Semi-quantitative |
| Measuring range |
<0.5 μg/L, 0.5-2 μg/L, 2-10 μg/L, >10 μg/L |
Reliability and reproducibility
Thermo Scientific B·R·A·H·M·S PCT-Q shows a high degree of reliability and reproducibility in the determination of serum/ plasma PCT concentrations. Semi-quantitative concentration ranges obtained with Thermo Scientific B·R·A·H·M·S PCT-Q correlate closely with the quantitative results obtained with Thermo Scientific B·R·A·H·M·S PCT LIA.
Thermo Scientific B·R·A·H·M·S PCT-Q achieves 90-92% of diagnostic sensitivity and 92-98% of diagnostic specificity when compared with the clinical use of Thermo Scientific B·R·A·H·M·S PCT LIA.
Test procedure
- Pipette 200 μL of serum/plasma into the round cavity. Document the time on the reference card.
- Incubate for 30 minutes at room temperature.
- After 30 minutes (max. 45 minutes), determine the PCT concentration range of the sample by comparing the colour intensity of the test band with the colour blocks of the reference card.
- No band or only test band visible: tests which show no control band are not valid and may not be evaluated.
- Only control band visible: tests which show only a control band are negatively valid. The PCT concentration is <0.5 μg/L.
- Control and test band visible: tests which show both a control band and a test band are positively valid.
