Chemiluminescence immunoassay (CLIA) for the determination of Procalcitonin (PCT) in human serum and plasma on the automated LIAISON and LIAISON XL random access analyser (DiaSorin).
Assay characteristics
The LIAISON B·R·A·H·M·S PCT assay is a two-step chemiluminescence immunoassay using two different highly specific monoclonal antibodies for the coating of the solid phase (magnetic particles) and for the tracer and can be distinguished by these characteristics:
| Sample material | Serum, plasma |
| Sample volume | 100 μL |
| Incubation time | 16 min |
| Measuring range | 0.02 up to 100 μg/L |
| Limit of Quantification (LoQ) | 0.04 μg/L |
| Analytical sensitivity (LoD) | 0.02 μg/L |
| High-dose hook | No high-dose hook effect detectable up to 2000 μg/L |
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Limit of Quantitation (LoQ) and Limit of Detection (LoD) values are rounded to 2 decimal places in alignment with the reported results for the assay. |
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